Proteome of Soybean Seed Exudates Contains Plant Defense-Related Proteins Active against the Root-Knot Nematode Meloidogyne incognita
详细信息 查看全文
- 作者：Raquel O. Rocha ; Janne K. S. Morais ; Jose T. A. Oliveira ; Hermogenes D. Oliveira ; Daniele O. B. Sousa ; Carlos Eduardo A. Souza ; Frederico B. Moreno ; Ana Cristina O. Monteiro-Moreira ; Jos茅 Dijair Antonino de Souza J煤nior ; Maria F. Grossi de S谩 ; Ilka M. Vasconcelos
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2015
- 出版时间：June 10, 2015
- 年：2015
- 卷：63
- 期：22
- 页码：5335-5343
- 全文大小：282K
- ISSN：1520-5118
文摘
Several studies have described the effects of seed exudates against microorganisms, but only few of them have investigated the proteins that have defensive activity particularly against nematode parasites. This study focused on the proteins released in the exudates of soybean seeds and evaluated their nematicidal properties against Meloidogyne incognita. A proteomic approach indicated the existence of 63 exuded proteins, including 尾-1,3-glucanase, chitinase, lectin, trypsin inhibitor, and lipoxygenase, all of which are related to plant defense. The presence of some of these proteins was confirmed by their in vitro activity. The soybean exudates were able to reduce the hatching of nematode eggs and to cause 100% mortality of second-stage juveniles (J2). The pretreatment of J2 with these exudates resulted in a 90% reduction of the gall number in tobacco plants. These findings suggest that the exuded proteins are directly involved in plant defense against soil pathogens, including nematodes, during seed germination.