A Golden Gate Modular Cloning Toolbox for Plants
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- 作者：Carola Engler ; Mark Youles ; Ramona Gruetzner ; Tim-Martin Ehnert ; Stefan Werner ; Jonathan D. G. Jones ; Nicola J. Patron ; Sylvestre Marillonnet
- 刊名：ACS Synthetic Biology
- 出版年：2014
- 出版时间：November 21, 2014
- 年：2014
- 卷：3
- 期：11
- 页码：839-843
- 全文大小：441K
- ISSN：2161-5063
文摘
Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed.