Formation of Silver Nanostructures by Rolling Circle Amplification Using Boranephosphonate-Modified Nucleotides
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- 作者：Camilla Russell ; Subhadeep Roy ; Saheli Ganguly ; Xiaoyan Qian ; Marvin H. Caruthers ; Mats Nilsson
- 刊名：Analytical Chemistry
- 出版年：2015
- 出版时间：July 7, 2015
- 年：2015
- 卷：87
- 期：13
- 页码：6660-6666
- 全文大小：459K
- ISSN：1520-6882
文摘
We investigate the efficiency of incorporation of boranephosphonate-modified nucleotides by phi29 DNA polymerase and present a simple method for forming large defined silver nanostructures by rolling circle amplification (RCA) using boranephosphonate internucleotide linkages. RCA is a linear DNA amplification technique that can use specifically circularized DNA probes for detection of target nucleic acids and proteins. The resulting product is a collapsed single-stranded DNA molecule with tandem repeats of the DNA probe. By substituting each of the natural nucleotides with the corresponding 5鈥?(伪-P-borano)deoxynucleosidetriphosphate, only a small reduction in amplification rate is observed. Also, by substituting all four natural nucleotides, it is possible to enzymatically synthesize a micrometer-sized, single-stranded DNA molecule with only boranephosphonate internucleotide linkages. Well-defined silver particles are then readily formed along the rolling circle product.