Single Step Isolation and Activation of Primary CD3+ T Lymphocytes Using Alcohol-Dispersed Electrospun Magnetic Nanofibers

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• 作者：Kwanghee Kim ; Hyo Jin An ; Seung-Hyun Jun ; Tae-Jin Kim ; Seon Ah Lim ; Gayoung Park ; Hyon Bin Na ; Yong Il Park ; Taeghwan Hyeon ; Cassian Yee ; Jeffrey A Bluestone ; Jungbae Kim ; Kyung-Mi Lee
• 刊名：Nano Letters
• 出版年：2012
• 出版时间：August 8, 2012
• 年：2012
• 卷：12
• 期：8
• 页码：4018-4024
• 全文大小：403K
• 年卷期：v.12,no.8(August 8, 2012)
• ISSN：1530-6992

文摘

Electrospun polymer nanofibers with entrapped magnetic nanoparticles (magnetic NP鈥揘F) represent a novel scaffold substrate that can be functionalized for single-step isolation and activation of specific lymphocyte subsets. Using a surface-embedded T cell receptor ligand/trigger (anti-CD3 monoclonal antibody), we demonstrate, as proof of principle, the use of magnetic NP鈥揘F to specifically isolate, enrich, and activate CD3⁺ T cells from a heterogeneous cell mixture, leading to preferential expansion of CD8⁺CD3⁺ T cells. The large surface area, adjustable antibody density, and embedded paramagnetic properties of the NP鈥揘F permitted enhanced activation and expansion; its use represents a strategy that is amenable to an efficient selection process for adoptive cellular therapy as well as for the isolation of other cellular subsets for downstream translational applications.

Keywords:

Electrospun magnetic nanofiber; CD4⁺ T lymphocytes; CD8⁺ T lymphocytes; anti-CD3 monoclonal antibody; immune cell therapy