Monolithic Integration of Two-Dimensional Liquid Chromatography鈭扖apillary Electrophoresis and Electrospray Ionization on a Microfluidic Device

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• 作者：Andrew G. Chambers ; J. Scott Mellors ; W. Hampton Henley ; J. Michael Ramsey
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：February 1, 2011
• 年：2011
• 卷：83
• 期：3
• 页码：842-849
• 全文大小：900K
• 年卷期：v.83,no.3(February 1, 2011)
• ISSN：1520-6882

文摘

A microfluidic device capable of two-dimensional reversed-phase liquid chromatography鈭抍apillary electrophoresis with integrated electrospray ionization (LC-CE-ESI) for mass spectrometry (MS) -based proteomic applications is described. Traditional instrumentation was used for the LC sample injection and delivery of the LC mobile phase. The glass microfabricated device incorporated a sample-trapping region and an LC channel packed with reversed-phase particles. Rapid electrokinetic injections of the LC effluent into the CE dimension were performed at a cross-channel intersection. The CE separation channel terminated at a corner of the square device, which functioned as an integrated electrospray tip. In addition to LC-CE-ESI, this device was used for LC-ESI without any instrumental modifications. To evaluate the system, LC-MS and LC-CE-MS analyses of protein digests were performed and compared.