Production and Elicitation of Benzalacetone and the Raspberry Ketone in Cell Suspension Cultures of Rubus idaeus

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• 作者：Seshu Pedapudi ; Chee-Kok Chin ; and Henrik Pedersen
• 刊名：Biotechnology Progress
• 出版年：2000
• 出版时间：June 2000
• 年：2000
• 卷：16
• 期：3
• 页码：346 - 349
• 全文大小：91K
• 年卷期：v.16,no.3(June 2000)
• ISSN：1520-6033

文摘

Production levels of p-coumaric acid (p-CA), p-hydroxyphenylbut-3-ene-2-one (benzalacetone), and p-hydroxyphenyl-2-butanone (raspberry ketone) were measured inraspberry cell suspension cultures to investigate metabolite dynamics in a short (two-step) pathway. Intracellular concentrations of benzalacetone and the raspberry ketonefluctuated during the time course of a normal batch culture cycle but showed higherlevels during periods of rapid growth. Cells elicited with the signal coupler methyljasmonate yielded a 2- to 3-fold increase in metabolite concentrations after 24 h. Theresults suggest that raspberry ketone production is rapidly inducible during periodsof high carbohydrate utilization. It is not an end product, however, and undergoesconversion to subsequent metabolites.