Quantification of Protodioscin and Rutin in Asparagus Shoots by LC/MS and HPLC Methods

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• 作者：Mingfu Wang ; Yaakov Tadmor ; Qing-Li Wu ; Chee-Kok Chin ; Stephen A. Garrison ; and James E. Simon
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2003
• 出版时间：October 8, 2003
• 年：2003
• 卷：51
• 期：21
• 页码：6132 - 6136
• 全文大小：85K
• 年卷期：v.51,no.21(October 8, 2003)
• ISSN：1520-5118

文摘

A liquid chromatography/mass spectrometry (LC/MS) method with selected ion monitoring wasdeveloped and validated to analyze the contents of protodioscin and rutin in asparagus. The distributionof rutin and protodioscin within the shoots was found to vary by location, with the tissue closest tothe rhizome found to be a rich source of protodioscin, at an average level of 0.025% tissue freshweight in the three tested lines, while the upper youngest shoot tissue contained the highest amountof rutin at levels of 0.03-0.06% tissue fresh weight. The lower portions of the asparagus shoots thatare discarded during grading and processing should instead be considered a promising source of anew value-added nutraceutical product.Keywords: Asparagus; protodioscin; rutin; LC/MS; quantification