New Valve and Bonding Designs for Microfluidic Biochips Containing Proteins

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• 作者：Chunmeng Lu ; Yubing Xie ; Yong Yang ; Mark M.-C. Cheng ; Chee-Guan Koh ; Yunling Bai ; and ; L. James Lee ; Yi-Je Juang
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：February 1, 2007
• 年：2007
• 卷：79
• 期：3
• 页码：994 - 1001
• 全文大小：628K
• 年卷期：v.79,no.3(February 1, 2007)
• ISSN：1520-6882

文摘

Two major concerns in the design and fabrication ofmicrofluidic biochips are protein binding on the channelsurface and protein denaturing during device assembly.In this paper, we describe new methods to solve theseproblems. A "fishbone" microvalve design based on theconcept of superhydrophobicity was developed to replacethe capillary valve in applications where the chip surfacerequires protein blocking to prevent nonspecific binding.Our experimental results show that the valve functionswell in a CD-like ELISA device. The packaging of biochipscontaining pre-loaded proteins is also a challenging tasksince conventional sealing methods often require the useof high temperatures, electric voltages, or organic solventsthat are detrimental to the protein activity. Using CO₂ gasto enhance the diffusion of polymer molecules near thedevice surface can result in good bonding at low temperatures and low pressure. This bonding method has littleinfluence on the activity of the pre-loaded proteins afterbonding.