Multi-mycotoxin Analysis of Finished Grain and Nut Products Using Ultrahigh-Performance Liquid Chromatography and Positive Electrospray Ionization鈥換uadrupole Orbital Ion Trap High-Resolution Mass Spec

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• 作者：Chia-Ding Liao ; Jon W. Wong ; Kai Zhang ; Paul Yang ; James B. Wittenberg ; Mary W. Trucksess ; Douglas G. Hayward ; Nathaniel S. Lee ; James S. Chang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：September 23, 2015
• 年：2015
• 卷：63
• 期：37
• 页码：8314-8332
• 全文大小：747K
• ISSN：1520-5118

文摘

Ultrahigh-performance liquid chromatography using positive electrospray ionization and quadrupole orbital ion trap high-resolution mass spectrometry was evaluated for analyzing mycotoxins in finished cereal and nut products. Optimizing the orbital ion trap mass analyzer in full-scan mode using mycotoxin-fortified matrix extracts gave mass accuracies, 未_M, of <卤2.0 ppm at 70鈥?00 full width at half maximum (FWHM) mass resolution (R_FWHM). The limits of quantitation were matrix- and mycotoxin-dependent, ranging from 0.02 to 11.6 渭g/kg. Mean recoveries and standard deviations for mycotoxins from acetonitrile/water extraction at their relevant fortification levels were 91 卤 10, 94 卤 10, 98 卤 12, 91 卤 13, 99 卤 15, and 93 卤 17% for corn, rice, wheat, almond, peanut, and pistachio, respectively. Nineteen mycotoxins with concentrations ranging from 0.3 (aflatoxin B₁ in peanut and almond) to 1175 渭g/kg (fumonisin B₁ in corn flour) were found in 35 of the 70 commercial grain and nut samples surveyed. Mycotoxins could be identified at 未_M < 卤5 ppm by identifying the precursor and product ions in full-scan MS and data-dependent MS/MS modes. This method demonstrates a new analytical approach for monitoring mycotoxins in finished grain and nut products.