Low Molecular Weight Chitosan Accelerates Glucagon-like Peptide-1 Secretion in Human Intestinal Endocrine Cells via a p38-Dependent Pathway

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• 作者：Shing Hwa Liu ; Ya Wen Huang ; Cheng Tien Wu ; Chen Yuan Chiu ; Meng Tsan Chiang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：May 22, 2013
• 年：2013
• 卷：61
• 期：20
• 页码：4855-4861
• 全文大小：337K
• 年卷期：v.61,no.20(May 22, 2013)
• ISSN：1520-5118

文摘

Chitosan is widely employed as a dietary supplement. Several studies have shown that chitosan possesses an antidiabetic effect. An important intestinal incretin hormone, glucagon-like peptide-1 (GLP-1), is also known to contribute to the amelioration of diabetes. This study investigated whether chitosan possesses an ability in GLP-1 synthesis and secretion in human intestinal cells. Low molecular weight chitosan (LMWC) significantly increases GLP-1 secretion in human intestinal endocrine cells (NCI-H716) in a dose-dependent manner. LMWC could also dose-dependently increase the mRNA expression of proglucagon, a GLP-1 precursor, but did not affect prohormone convertase 3 (PC 3) mRNA expression. LMWC effectively increased the phosphorylation of mitogen-activated protein kinases (MAPK)-p38 and c-Jun N-terminal kinases (JNK), but not extracellular-signal-regulated kinases (ERK). An inhibitor of p38, but not JNK and ERK, significantly reversed the LMWC-increased proglucagon expression. Taken together, LMWC accelerates proglucagon expression and GLP-1 secretion through a p38/MAPK-dependent signaling pathway. These findings suggest that LMWC may provide a strategy for diabetes therapy.

Keywords:

chitosan; glucagon-like peptide-1; proglucagon; p38 MAPK; intestine