Alkali-Soluble Polysaccharides of Rhizoclonium riparium Alga Induce IL-1 Gene Expression via Protein Kinase Signaling Pathways
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- 作者：Hsien-Yeh Hsu ; Kuo-Feng Hua ; Yu-Chang Su ; Li-Chieh Chu ; Shi-Chi Su ; Huan-Wen Chiu ; Chi-Huey Wong ; Shui-Tein Chen ; Chu-Wen Shieh ; Su-Sing Yang ; Ya-Mai Chen ; and Louis Kuoping Chao
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2006
- 出版时间：May 17, 2006
- 年：2006
- 卷：54
- 期：10
- 页码：3558 - 3565
- 全文大小：318K
- 年卷期：v.54,no.10(May 17, 2006)
- ISSN：1520-5118
文摘
Fortification of aquaculture foodstuff with various algae may improve the resistance of certain fish orshrimp to diseases and, as a routine procedure, has become ever more popular and, seemingly,important. Herein, we isolated certain alkali-soluble polysaccharides from a Rhizoclonium ripariumalga (RASP), polysaccharides that can be separated into two different groups on the basis of thepolysaccharide's molecular weight. Using gas chromatography-mass spectometry analysis, we foundthat the major monosaccharides constituting the higher molecular-weight group of RASP weregalactose (41.99%), glucose (34.53%), xylose (20.24%), and mannose (3.24%). Using a murine-derived macrophage cell line J774A.1, we found that polysaccharide constituents of the highermolecular-weight group of RASP were able to induce interleukin-1beta (IL-1) gene expression viaprotein kinase-mediated signal transduction pathways. In essence, we found that c-Jun N-terminalkinase (JNK) and p38 mitogen-activated protein kinase (p38), but not extracellular signal-regulatedkinase (ERK), play an important role in the regulation of IL-1 gene expression in RASP-stimulatedJ774A.1 cells. To the best of our knowledge, this is the first occasion that polysaccharides from R.riparium have been demonstrated to exert immunomodulation properties by the induction of IL-1within macrophages. Our current results provide support for the possible use of R. riparium as anadditive to various food/foodstuff, to modulate the immune response of humans or certain animals.Keywords: Algae; polysaccharide; macrophage; IL-1; mitogen-activated protein kinases