Recombinant Porcine Lactoferrin Expressed in the Milk of Transgenic Mice Enhances Offspring Growth Performance
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- 作者：Shinn-Chih Wu ; Hsiao-Ling Chen ; Chih-Ching Yen ; Meng-Fu Kuo ; Tien-Shuh Yang ; Shih-Rong Wang ; Chung-Nan Weng ; Chuan-Mu Chen ; Winston T. K. Cheng
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：June 13, 2007
- 年：2007
- 卷：55
- 期：12
- 页码：4670 - 4677
- 全文大小：675K
- 年卷期：v.55,no.12(June 13, 2007)
- ISSN：1520-5118
文摘
The European Commission has proposed a permanent ban on the use of antibiotics as an ingredientin animal feed to promote growth. Lactoferrin is a globular multifunctional protein that has been shownto play a role in iron absorption and to have antimicrobial and anti-inflammatory activities. Therefore,lactoferrin may serve as a nontherapeutic alternative to antibiotics in livestock husbandry. As a pilotstudy toward this goal, transgenic mice have been generated harboring a porcine lactoferrin (pLF)gene driven by the mammary gland-specific promoter of the bovine 
-lactalbumin (LA) gene. TheLA-pLF hybrid gene was confirmed to have been successfully integrated and transmitted stablythrough the germ-line in 9 (5 females and 4 males) of 14 transgenic founders. In the female progeniesof six lines analyzed, the transgene copy numbers ranged from 1 to 20 with 1-4 integration sites.Significant levels of pLF protein in milk ranging from 40 to 106 
g/mL with physical characteristicssimilar to those of native pLF in sow's milk were achieved in three of the transgenic lines obtained.Tissue- and stage-specific pLF expressions were restricted to the mammary gland of the transgenicfemale mice during lactation. It was further demonstrated that the growth performance of animalpups is enhanced by directly feeding the genetically engineered milk containing enriched pLF proteinin transgenic mice. Furthermore, this enhanced growth performance in suckling mice was proportionalto the concentration of pLF present in milk.
-lactalbumin (LA) gene. TheLA-pLF hybrid gene was confirmed to have been successfully integrated and transmitted stablythrough the germ-line in 9 (5 females and 4 males) of 14 transgenic founders. In the female progeniesof six lines analyzed, the transgene copy numbers ranged from 1 to 20 with 1-4 integration sites.Significant levels of pLF protein in milk ranging from 40 to 106 g/mL with physical characteristicssimilar to those of native pLF in sow's milk were achieved in three of the transgenic lines obtained.Tissue- and stage-specific pLF expressions were restricted to the mammary gland of the transgenicfemale mice during lactation. It was further demonstrated that the growth performance of animalpups is enhanced by directly feeding the genetically engineered milk containing enriched pLF proteinin transgenic mice. Furthermore, this enhanced growth performance in suckling mice was proportionalto the concentration of pLF present in milk.