The Perturbation of Tryptophan Fluorescence by Phenylalanine to Alanine Mutations Identifies the Hydrophobic Core in a Subset of Bacterial Ig-like Domains

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• 作者：Rajeev Raman ; Christopher P. Ptak ; Ching-Lin Hsieh ; Robert E. Oswald ; Yung-Fu Chang ; Yogendra Sharma
• 刊名：Biochemistry
• 出版年：2013
• 出版时间：July 9, 2013
• 年：2013
• 卷：52
• 期：27
• 页码：4589-4591
• 全文大小：193K
• 年卷期：v.52,no.27(July 9, 2013)
• ISSN：1520-4995

文摘

Many host鈥損arasite interactions are mediated via surface-exposed proteins containing bacterial immunoglobulin-like (Big) domains. Here, we utilize the spectral properties of a conserved Trp to provide evidence that, along with a Phe, these residues are positioned within the hydrophobic core of a subset of Big_2 domains. The mutation of the Phe to Ala decreases Big_2 domain stability and impairs the ability of LigBCen2 to bind to the host protein, fibronectin.