Cloning and Characterization of a Plant Defensin VaD1 from Azuki Bean

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• 作者：Gan-Hong Chen ; Ming-Pin Hsu ; Chi-Hsing Tan ; Hsien-Yi Sung ; C. George Kuo ; Ming-Jen Fan ; Huei-Mei Chen ; Shu Chen ; and Ching-San Chen
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：February 23, 2005
• 年：2005
• 卷：53
• 期：4
• 页码：982 - 988
• 全文大小：298K
• 年卷期：v.53,no.4(February 23, 2005)
• ISSN：1520-5118

文摘

A recombinant mungbean defensin VrD1 was previously shown to exhibit antifungal and bruchid-resistant activity. To study the function and regulation of VrD1, genomic DNAs of plant defensinswere isolated from Vigna radiata VC6089A and azuki bean Vigna angularis Kao Hsiung No. 6. Theazuki bean defensin genomic DNA VaD1 was sequenced and converted to VaD1 cDNA. VaD1defensin was purified from Vigna angularis Kao Hsiung No. 6 to apparent homogeneity. The completeamino acid sequence of the purified VaD1 was determined and was found to be exactly the same asthe sequence deduced from VaD1 cDNA. VaD1 is a basic protein containing 46 amino acids withfour conserved disulfide bonds and shares high sequence homology (78.3%) with VrD1. VaD1 inhibitedthe growth of Fusarium oxysporum, Fusarium oxysporum f. sp. pisi, Staphylococcus epidermidis,and Salmonella typhimurium. VaD1 also inhibited in vitro protein synthesis and bruchid larvaldevelopment, but was less active than the recombinant VrD1.Keywords: Antimicrobial activity; azuki bean (Vigna angularis); bruchid resistance; defensin; proteinsynthesis inhibition