Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide
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- 作者：Noelia Rubio ; Kuo-Ching Mei ; Rebecca Klippstein ; Pedro M. Costa ; Naomi Hodgins ; Julie Tzu-Wen Wang ; Frederic Festy ; Vincenzo Abbate ; Robert C. Hider ; Ka Lung Andrew Chan ; Khuloud T. Al-Jamal
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2015
- 出版时间：September 2, 2015
- 年：2015
- 卷：7
- 期：34
- 页码：18920-18923
- 全文大小：302K
- ISSN：1944-8252
文摘
Polyethylene glycol-functionalized nanographene oxide (PEGylated n-GO) was synthesized from alkyne-modified n-GO, using solvent-free click-mechanochemistry, i.e., copper(I)-catalyzed azide鈥揳lkyne cycloaddition (CuAAC). The modified n-GO was subsequently conjugated to a mucin 1 receptor immunoglobulin G antibody (anti-MUC1 IgG) via thiol鈥揺ne coupling reaction. n-GO derivatives were characterized with Fourier-transformed infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), Bradford assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and atomic force microscopy (AFM). Cell targeting was confirmed in vitro in MDA-MB-231 cells, either expressing or lacking MUC1 receptors, using flow cytometry, confocal laser scanning microscopy (CLSM) and multiphoton (MP) fluorescence microscopy. Biocompatibility was assessed using the modified lactate dehydrongenase (mLDH) assay.