Investigating the Spatial Distribution of Integrin 尾1 in Patterned Human Mesenchymal Stem Cells Using Super-Resolution Imaging

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• 作者：Ajay Tijore ; Srivats Hariharan ; Haiyang Yu ; Chee Ren Ivan Lam ; Feng Wen ; Chor Yong Tay ; Sohail Ahmed ; Lay Poh Tan
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2014
• 出版时间：September 24, 2014
• 年：2014
• 卷：6
• 期：18
• 页码：15686-15696
• 全文大小：711K
• ISSN：1944-8252

文摘

Lineage commitment of human mesenchymal stem cells (hMSCs) could be directed through micro/nanopatterning of the extracellular matrix (ECM) between cells and substrate. Integrin receptors, integrator of the ECM and cell cytoskeleton, function as molecular bridges linking cells to different biophysical cues translated from patterned ECM. Here we report the distinct recruitment of active integrin 尾₁ (ITG-尾₁) in hMSCs when they were committed toward the cardiomyogenic lineage on a micropatterned surface. In addition, a systematic study of the distribution of ITG-尾₁ was performed on focal adhesions (FAs) using a direct stochastic optical reconstruction microscopy (dSTORM) technique, a super-resolution imaging technique to establish the relationship between types of integrin expression and its distribution pattern that are associated with cardiomyogenic differentiation of hMSCs. We ascertained that elongated FAs of ITG-尾₁ expressed in patterned hMSCs were more prominent than FAs expressed in unpatterned hMSCs. However, there was no significant difference observed between the widths of FAs from both experimental groups. It was found in patterned hMSCs that the direction of FA elongation coincides with cell orientation. This phenomenon was however not observed in unpatterned hMSCs. These results showed that the biophysical induction methods like FAs patterning could selectively induce hMSCs lineage commitment via integrin-material interaction.

Keywords:

hMSCs; integrin 尾₁; focal adhesion; cardiomyogenic differentiation; super-resolution imaging