Development of a Sensitive High-Performance Liquid Chromatography-Diode Array Detection Assay for the Detection and Quantification of the Beauveria Metabolite Oosporein from Submerged Culture B

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• 作者：Christoph Seger ; Sonja Sturm ; Tobias Lä ; ngle ; Waltraud Wimmer ; Hermann Stuppner ; and Hermann Strasser
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：March 9, 2005
• 年：2005
• 卷：53
• 期：5
• 页码：1364 - 1369
• 全文大小：62K
• 年卷期：v.53,no.5(March 9, 2005)
• ISSN：1520-5118

文摘

A sensitive high-performance liquid chromatography-diode array detection (HPLC-DAD) assay isdescribed for the detection and quantification of the Beauveria metabolite oosporein from fungal culturebroth and two biocontrol agent formulations. In all cases, analyte recovery was achieved with a Britton-Robinson buffer system at pH 5.5 diluted with methanol 3:7 (v/v) (BR5.5-MeOH). The HPLC-DADassay, using a binary solvent gradient with acidic modifiers and detecting the metabolite at 287 nm,showed linearity over 3 orders of magnitude and a limit of detection (LOD) of 6.0 ± 2.3 g ofoosporein/L of BR5.5-MeOH. The oosporein content of the representative fungal culture broth samplesand two Beauveria formulations (Melocont-Pilzgerste and Melocont-WP) was found to be 504.7 ±13.6 mg of oosporein/L of culture filtrate, 7.4 ± 0.6 mg of oosporein/kg of Melocont-Pilzgerste, and38.2 ± 1.3 mg of oosporein/kg of Melocont-WP with recovery rates of 93 ± 2, 99 ± 8, and 92 ± 3%,respectively.Keywords: HPLC-DAD; Beauveria brongniartii; oosporein; hydroxybenzoquinone derivative; analyteadsorption; biological pest control formulations; risk assessment