RCADiA: Simple Automation Platform for Comparative Multidimensional Protein Identification Technology
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- 作者：Aaron O. Bailey ; Timothy M. Miller ; Meng-Qiu Dong ; Christine Vande Velde ; Don W. Cleveland ; John R. Yates
- 刊名：Analytical Chemistry
- 出版年：2007
- 出版时间：August 15, 2007
- 年：2007
- 卷：79
- 期：16
- 页码：6410 - 6418
- 全文大小：719K
- 年卷期：v.79,no.16(August 15, 2007)
- ISSN：1520-6882
文摘
Multidimensional liquid chromatography in combinationwith tandem mass spectrometry has been used to analyzea variety of biological structures including protein complexes. Incorporating this approach with autosamplingdevices presents a number of problems including decreased sensitivity due to exposure to extra surfaces,carryover from run to run, and increased dead volume.We developed a device, termed Radial Column Array forDistribution and Automation (RCADiA), to automatemultiple MuDPIT experiments while eliminating many ofthese problems and maintaining a high resolution andsensitive analysis. The design, which places each sampledownstream of any common fluid path, presents a low riskof carryover between successive analyses. Beyond theconvenience of automation, the RCADiA platform alsoproduces data of similar quality to the standard methodof performing individual MuDPIT experiments. We demonstrate this device by performing a comparative analysisof mitochondria enriched from rat liver and spinal cord.