Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment

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• 作者：Lynn M. McGregor ; David J. Gorin ; Christoph E. Dumelin ; David R. Liu
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：November 10, 2010
• 年：2010
• 卷：132
• 期：44
• 页码：15522-15524
• 全文大小：241K
• 年卷期：v.132,no.44(November 10, 2010)
• ISSN：1520-5126

文摘

Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein−ligand pairs out of 67599 possible sequences.