Multiplexed Sandwich Immunoassays Using Electrochemiluminescence Imaging Resolved at the Single Bead Level

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• 作者：Frdrique Deiss ; Christopher N. LaFratta ; Matthew Symer ; Timothy M. Blicharz ; Neso Sojic ; David R. Walt
• 刊名：Journal of the American Chemical Society
• 出版年：2009
• 出版时间：May 6, 2009
• 年：2009
• 卷：131
• 期：17
• 页码：6088-6089
• 全文大小：146K
• 年卷期：v.131,no.17(May 6, 2009)
• ISSN：1520-5126

文摘

A new class of bead-based microarray that uses electrogenerated chemiluminescence (ECL) as a readout mechanism to detect multiple antigens simultaneously is presented. This platform demonstrates the possibility of performing highly multiplexed assays using ECL because all the individual sensing beads in the array are simultaneously imaged and individually resolved by ECL. Duplex and triplex assay results are demonstrated as well as a cross reactivity study.