Isolation, Structure Characterization, and Immunomodulating Activity of a Hyperbranched Polysaccharide from the Fruiting Bodies of Ganoderma sinense
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- 作者：Xiao-Qiang Han ; Ben Chung Lap Chan ; Cai-Xia Dong ; Yin-Hua Yang ; Chun-Hay Ko ; Grace Gar-Lee Yue ; Dan Chen ; Chun-Kwok Wong ; Clara Bik-San Lau ; Peng-Fei Tu ; Pang-Chui Shaw ; Kwok-Pui Fung ; Ping-Chung Leung ; Wen-Luan Hsiao ; Quan-Bin Han
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：May 2, 2012
- 年：2012
- 卷：60
- 期：17
- 页码：4276-4281
- 全文大小：311K
- 年卷期：v.60,no.17(May 2, 2012)
- ISSN：1520-5118
文摘
A polysaccharide (GSP-6B) with a molecular mass of 1.86 脳 106 Da was isolated from the fruiting bodies of Ganoderma sinense. Chemical composition analysis, methylation analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1鈫?)-linked-尾-d-glucopyranosyl residues, bearing branches at the O-3 position of every two sugar residues along the backbone. The side chains contain (1鈫?)-linked-尾-d-glucopyranosyl residues, (1鈫?)-linked-尾-d-glucopyranosyl residues, and nonreducing end 尾-d-glucopyranosyl residues. An in vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the release of IL-1尾 and TNF-伪 in human peripheral blood mononuclear cell (PBMC) and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate dendritic cells (DC) by stimulating the secretion of IL-12 and IL-10 from DC.