Study on Cast Membranes and Electrospun Nanofibers Made from Keratin/Fibroin Blends

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• 作者：Marina Zoccola ; Annalisa Aluigi ; Claudia Vineis ; Claudio Tonin ; Franco Ferrero ; Marco G. Piacentino
• 刊名：Biomacromolecules
• 出版年：2008
• 出版时间：October 13, 2008
• 年：2008
• 卷：9
• 期：10
• 页码：2819-2825
• 全文大小：431K
• 年卷期：v.9,no.10(October 13, 2008)
• ISSN：1526-4602

文摘

Keratin regenerated from wool and fibroin regenerated from silk were mixed in different proportions using formic acid as the common solvent. Both solutions were cast to obtain films and electrospun to produce nanofibers. Scanning electron microscopy investigation showed that, for all electrospun blends (except for 100% keratin where bead defects are present), the fiber diameter of the mats ranged from 900 (pure fibroin) to 160 nm (pure keratin). FTIR and DSC analysis showed that the secondary structure of the proteins was influenced by the blend ratios and the process used (casting or electrospinning). Prevalence of β-sheet supramolecular structures was observed in the films, while proteins assembled in α-helix/random coil structures were observed in nanofibers. Higher solution viscosity, thinner filaments, and differences in the thermal and structural properties were observed for the 50/50 blend because of the enhanced interactions between the proteins.