Novel Microwave-Assisted Solvothermal Synthesis of NaYF4:Yb,Er Upconversion Nanoparticles and Their Application in Cancer Cell Imaging

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• 作者：Congcong Mi ; Zhenhuang Tian ; Can Cao ; Zhijia Wang ; Chuanbin Mao ; Shukun Xu
• 刊名：Langmuir
• 出版年：2011
• 出版时间：December 6, 2011
• 年：2011
• 卷：27
• 期：23
• 页码：14632-14637
• 全文大小：871K
• 年卷期：v.27,no.23(December 6, 2011)
• ISSN：1520-5827

文摘

This work reports the novel microwave-assisted solvothermal synthesis and structural, topographic, spectroscopic characterization of NaYF₄:Yb,Er upconversion nanoparticles (UCNPs) as well as their application in the labeling of HeLa cells. The nanoparticles were prepared in ethylene glycol, with rare earth acetates as precursor and NH₄F and NaCl as the fluorine and sodium sources. X-ray diffraction, transmission electron microscopy, and luminescence spectroscopy were applied to characterize the nanoparticles. Experimental results showed that the microwave-assisted solvothermal method is an effective approach to create highly crystalline, strongly luminescent UCNPs at a lower temperature (160 掳C) and within a significantly shortened reaction time (only 1 h) compared to the traditional methods. The effect of fluorine source on the optical properties of UCNPs was investigated by using NH₄F, NH₄HF₂, NaF, and 1-butyl-3-methylimidazolium tetrafluoroborate (BmimBF₄) as different fluorine sources; NH₄F proved to be the best one, making the luminescent intensity increase at least 2 orders of magnitude. The UCNPs with four different colors (green, yellow, orange, and cyan) were successfully obtained. After being modified with amino groups and coupled with CEA-8 antibody, the obtained nanoparticles were successfully applied in the specific fluorescent immunolabeling and imaging of HeLa cells to further verify their function as a marker in immunolabeling.