End-Functionalized Phosphorylcholine Methacrylates and their Use in Protein Conjugation

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• 作者：Debasis Samanta ; Samantha McRae ; Beth Cooper ; Yunxia Hu ; Todd EmrickJeanne Pratt ; Stephen A. Charles
• 刊名：Biomacromolecules
• 出版年：2008
• 出版时间：October 13, 2008
• 年：2008
• 卷：9
• 期：10
• 页码：2891-2897
• 全文大小：209K
• 年卷期：v.9,no.10(October 13, 2008)
• ISSN：1526-4602

文摘

Polymer−protein conjugation was performed using N-hydroxysuccinimide and aldehyde-terminated zwitterionic polymers, and the resulting polymer−protein conjugates were characterized by gel electrophoresis and fast protein liquid chromatography. Methacryloyloxyethyl phosphorylcholine (MPC) polymers were prepared by atom transfer radical polymerization in which the requisite functional end-groups for protein conjugation were embedded within the polymerization initiators. These phosphorylcholine polymers were conjugated to lysozyme as a model protein, as well as two therapeutic proteins, granulocyte colony stimulating factor (G-CSF) and erythropoietin (EPO). These MPC polymer−protein conjugates represent alternatives to PEGylated proteins, with the potential to provide improved efficacy in a therapeutic treatment relative to the protein itself.