Structural Consequences of Cysteinylation of Cu/Zn-Superoxide Dismutase
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- 作者：Jared R. Auclair ; Heather R. Brodkin ; J. Alejandro D鈥橝quino ; Gregory A. Petsko ; Dagmar Ringe ; Jeffrey N. Agar
- 刊名：Biochemistry
- 出版年：2013
- 出版时间：September 10, 2013
- 年：2013
- 卷：52
- 期：36
- 页码：6145-6150
- 全文大小：275K
- 年卷期：v.52,no.36(September 10, 2013)
- ISSN：1520-4995
文摘
The metalloenzyme Cu/Zn-superoxide dismutase (SOD1) catalyzes the reduction of superoxide anions into molecular oxygen and hydrogen peroxide. Hydrogen peroxide can oxidize SOD1, resulting in aberrant protein conformational changes, disruption of SOD1 function, and DNA damage. Cells may have evolved mechanisms of regulation that prevent such oxidation. We observed that cysteinylation of cysteine 111 (Cys111) of SOD1 prevents oxidation by peroxide (DOI 10.1021/bi4006122). In this article, we characterize cysteinylated SOD1 using differential scanning fluorometry and X-ray crystallography. The stoichiometry of binding was one cysteine per SOD1 dimer, and there does not appear to be free volume for a second cysteine without disrupting the dimer interface. Much of the three-dimensional structure of SOD1 is unaffected by cysteinylation. However, local conformational changes are observed in the cysteinylated monomer that include changes in conformation of the electrostatic loop (loop VII; residues 133鈥?44) and the dimer interface (loop VI; residues 102鈥?15). In addition, our data shows how cysteinylation precludes oxidation of cysteine 111 and suggests possible cross-talk between the dimer interface and the electrostatic loop.