Effective Targeting of DC-SIGN by 伪-Fucosylamide Functionalized Gold Nanoparticles

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• 作者：Daniela Arosio ; Fabrizio Chiodo ; Jos茅 J. Reina ; Marcello Marelli ; Soledad Penad茅s ; Yvette van Kooyk ; Juan J. Garcia-Vallejo ; Anna Bernardi
• 刊名：Bioconjugate Chemistry
• 出版年：2014
• 出版时间：December 17, 2014
• 年：2014
• 卷：25
• 期：12
• 页码：2244-2251
• 全文大小：376K
• ISSN：1520-4812

文摘

Dendritic Cells (DCs), the most potent antigen-presenting cells, play a critical role in the detection of invading pathogens, which are recognized also by multiple carbohydrate-specific receptors. Among them, DC-SIGN is one of the best characterized, with high-mannose and Lewis-type glycan specificity. In this study, we present a potent DC-SIGN targeting device developed using gold nanoparticles functionalized with 伪-fucosyl-尾-alanyl amide. The nanoparticles bound to cellular DC-SIGN and induced internalization as effectively as similar particles coated with comparable amounts of Lewis^X oligosaccharide. They were found to be neutral toward dendritic cell maturation and IL-10 production, thus envisaging a possible use as targeted imaging tools and antigen delivery devices.