Noninvasive Detection of Inflammatory Changes in White Adipose Tissue by Label-Free Raman Spectroscopy
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- 作者：Abigail S. Haka ; Erika Sue ; Chi Zhang ; Priya Bhardwaj ; Joshua Sterling ; Cassidy Carpenter ; Madeline Leonard ; Maryem Manzoor ; Jeanne Walker ; Jose O. Aleman ; Daniel Gareau ; Peter R. Holt ; Jan L. Breslow ; Xi Kathy Zhou ; Dilip Giri ; Monica Morrow ; Neil Iyengar ; Ishan Barman ; Clifford A. Hudis ; Andrew J. Dannenberg
- 刊名：Analytical Chemistry
- 出版年：2016
- 出版时间：February 16, 2016
- 年：2016
- 卷：88
- 期：4
- 页码：2140-2148
- 全文大小：503K
- ISSN：1520-6882
文摘
White adipose tissue inflammation (WATi) has been linked to the pathogenesis of obesity-related diseases, including type 2 diabetes, cardiovascular disease, and cancer. In addition to the obese, a substantial number of normal and overweight individuals harbor WATi, putting them at increased risk for disease. We report the first technique that has the potential to detect WATi noninvasively. Here, we used Raman spectroscopy to detect WATi with excellent accuracy in both murine and human tissues. This is a potentially significant advance over current histopathological techniques for the detection of WATi, which rely on tissue excision and, therefore, are not practical for assessing disease risk in the absence of other identifying factors. Importantly, we show that noninvasive Raman spectroscopy can diagnose WATi in mice. Taken together, these results demonstrate the potential of Raman spectroscopy to provide objective risk assessment for future cardiometabolic complications in both normal weight and overweight/obese individuals.