Size and Density of Fibers in Fibrin and Other Filamentous Networks from Turbidimetry: Beyond a Revisited Carr鈥揌ermans Method, Accounting for Fractality and Porosity

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• 作者：Fabio Ferri ; Gabriele Re Calegari ; Matteo Molteni ; Barbara Cardinali ; Davide Magatti ; Mattia Rocco
• 刊名：Macromolecules
• 出版年：2015
• 出版时间：August 11, 2015
• 年：2015
• 卷：48
• 期：15
• 页码：5423-5432
• 全文大小：525K
• ISSN：1520-5835

文摘

The Carr鈥揌ermans method [ Macromolecules 1978, 11, 46鈭?0], often used for determining the fibers diameter d and density 蟻 in fibrin or other filamentous networks from turbidity data, is found to be remarkably inaccurate when the system鈥檚 mass fractal dimension D_m is >1. An expanded approach based on the knowledge of the system D_m and pore size 尉, which can be accurately recovered from low-angle elastic light scattering data or estimated from confocal microscopy, is proposed. By fitting the turbidity data with a function obtained by numerically integrating the fibrin-optimized scattering form factor of a network of cylindrical elements, both d and 蟻 can be independently recovered. Numerical simulations were employed to validate the reliability and accuracy of the method, which is then applied to evolving fibrin gels data. More in general, this method is extendible to the analysis of other filamentous networks that can be represented as ensembles of cylindrical elements.