Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid “Invasion”Andrea L. Stadler, Dazhi Sun, Mathew M. Maye, Daniel van der Lelie, and Oleg Gang pp 2467–2474Publication Date (Web): March 9, 2011 (Article)DOI: 10.1021/nn101355nAbstract

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• 刊名：ACS Nano
• 出版年：2011
• 出版时间：April 26, 2011
• 年：2011
• 卷：5
• 期：4
• 全文大小：1039K
• 年卷期：0
• ISSN：1936-086X

文摘

We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)−DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.