The acaricidal activity of materials derived from the rhizome of
Cnidium officinale against adults of
Dermatophagoides farinae and
Dermatophagoides pteronyssinus was examined using direct contactapplication and fumigation methods and compared with that of benzyl benzoate and
N,
N-diethyl-
m-toluamide (DEET). The active constituent of the
Cnidium rhizome was identified as butylidenephthalideby spectroscopic analyses. Responses varied with dose. On the basis of 24-h LD
50 values, theacaricidal activity of butylidenephthalide (6.77
g/cm
2) against
D. farinae adults was comparable tothat of benzyl benzoate (8.54
g/cm
2). Very low activity was observed with DEET (37.59
g/cm
2).Against
D. pteronyssinus adults, butylidenephthalide (6.46
g/cm
2) and benzyl benzoate (6.68
g/cm
2) were equitoxic. DEET (17.98
g/cm
2) was relatively inactive. The typical poisoning symptom ofbutylidenephthalide was lethargy of treated mites, leading to death without knockdown, whereas benzylbenzoate and DEET caused death following uncoordinated behavior. In a fumigation test with bothmite species, butylidenephthalide was much more effective in closed containers than open ones.Naturally occurring
C.
officinale rhizome-derived materials merit further study as potential house dustmite control agents or lead compounds.Keywords: Natural acaricide; natural fumigant;
Dermatophagoides farinae;
Dermatophagoides pteronyssinus;
Cnidium officinale; butylidenephthalide; mode of action; poisoning symptom