Installing Logic Gates in Permeability Controllable Polyelectrolyte-Carbon Nitride Films for Detecting Proteases and Nucleases

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• 作者：Lichan Chen ; Xiaoting Zeng ; Anirban Dandapat ; Yuwu Chi ; Donghwan Kim
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：September 1, 2015
• 年：2015
• 卷：87
• 期：17
• 页码：8851-8857
• 全文大小：414K
• ISSN：1520-6882

文摘

Proteases and nucleases are enzymes heavily involved in many important biological processes, such as cancer initiation, progression, and metastasis; hence, they are indicative of potential diagnostic biomarkers. Here, we demonstrate a new label free and sensitive electrochemiluminescent (ECL) sensing strategy for protease and nuclease assays that utilize target-triggered desorption of programmable polyelectrolyte films assembled on graphite-like carbon nitride (g-C₃N₄) film to regulate the diffusion flux of a coreactant. Furthermore, we have built Boolean logic gates OR and AND into the polyelectrolyte films, capable of simultaneously sensing proteases and nucleases in a complicated system by breaking it into simple functions. The developed intelligent permeability controlled enzyme sensor may prove valuable in future medical diagnostics.