Quinoline-Based Fluorescent Probe for Ratiometric Detection of Lysosomal pH
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- 作者：Guoping Li ; Dongjian Zhu ; Lin Xue ; Hua Jiang
- 刊名：Organic Letters
- 出版年：2013
- 出版时间：October 4, 2013
- 年：2013
- 卷：15
- 期：19
- 页码：5020-5023
- 全文大小：268K
- 年卷期：v.15,no.19(October 4, 2013)
- ISSN：1523-7052
文摘
A new pH-responsive fluorescent probe has been reported based on protonation-activable resonance charge transfer. In aqueous solution, probe PQ-Lyso exhibits ratiometric detection of pH changes with a large hypsochromic shift of 76 nm and remarkable changes in the fluorescence intensity ratio (R = F494聽nm/F570聽nm, R/R0 = 105). Furthermore, PQ-Lyso, which is localized to lysosomes in living cells, can calibrate lysosomal pH using fluorescence ratiometry.