Shear Flow Induced Changes in Apolipoprotein C-II Conformation and Amyloid Fibril Formation

详细信息    查看全文

• 作者：Chai Lean Teoh ; Innocent B. Bekard ; Peter Asimakis ; Michael D. W. Griffin ; Timothy M. Ryan ; Dave E. Dunstan ; Geoffrey J. Howlett
• 刊名：Biochemistry
• 出版年：2011
• 出版时间：May 17, 2011
• 年：2011
• 卷：50
• 期：19
• 页码：4046-4057
• 全文大小：1121K
• 年卷期：v.50,no.19(May 17, 2011)
• ISSN：1520-4995

文摘

The misfolding and self-assembly of proteins into amyloid fibrils that occur in several debilitating diseases are affected by a variety of environmental factors, including mechanical factors associated with shear flow. We examined the effects of shear flow on amyloid fibril formation by human apolipoprotein C-II (apoC-II). Shear fields (150, 300, and 500 s^鈥?) accelerated the rate of apoC-II fibril formation (1 mg/mL) approximately 5鈥?0-fold. Fibrils produced at shear rates of 150 and 300 s^鈥? were similar to the twisted ribbon fibrils formed in the absence of shear, while at 500 s^鈥?, tangled ropelike structures were observed. The mechanism of the shear-induced acceleration of amyloid fibril formation was investigated at low apoC-II concentrations (50 渭g/mL) where fibril formation does not occur. Circular dichroism and tryptophan fluorescence indicated that shear induced an irreversible change in apoC-II secondary structure. Fluorescence resonance energy transfer experiments using the single tryptophan residue in apoC-II as the donor and covalently attached acceptors showed that shear flow increased the distance between the donor and acceptor molecules. Shear-induced higher-order oligomeric species were identified by sedimentation velocity experiments using fluorescence detection, while fibril seeding experiments showed that species formed during shear flow are on the fibril formation pathway. These studies suggest that physiological shear flow conditions and conditions experienced during protein manufacturing can exert significant effects on protein conformation, leading to protein misfolding, aggregation, and amyloid fibril formation.