Multifunctional Janus Hematite鈥揝ilica Nanoparticles: Mimicking Peroxidase-Like Activity and Sensitive Colorimetric Detection of Glucose

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• 作者：Chang Lu ; Xiangjiang Liu ; Yunfeng Li ; Fang Yu ; Longhua Tang ; Yanjie Hu ; Yibin Ying
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：July 22, 2015
• 年：2015
• 卷：7
• 期：28
• 页码：15395-15402
• 全文大小：447K
• ISSN：1944-8252

文摘

The design and engineering of multifunctional nanostructures with multiple components and synergistic properties are in urgent demand for variety of acceptable biosensing platforms, enabling users to fulfill multiple tasks in a single nanosystem. Herein, we report using an asymmetric hematite鈥搒ilica hybrid of Janus 纬-Fe₂O₃/SiO₂ nanoparticles (JFSNs) as a multifunctional biosensing platform for sensitive colorimetric detection of H₂O₂ and glucose. It was demonstrated that JFSNs exhibit an intrinsic peroxidase-like catalytic activity. Compared with natural enzyme, JFSNs nanoenzymes could be used over a wider range of pH and temperatures and were more stable over time. Importantly, besides its excellent catalytic activity, the asymmetric properties of the Janus nanoparticle enable it to form the multiple functional utilities for various biosensing applications, including the ease of surface modification without deactivation of catalytic activity and recoverable use by magnetic separation. Thus, we utilized JFSNs with glucose oxidase (GOx) immobilization for glucose-sensitive colorimetric detection, which exhibited both catalytic activity of glucose oxidase and peroxidase with high selectivity and acceptable reproducibility. By combining these two analysis systems into Janus particles, an all-in-one and reusable sensor for blood glucose was formed and has the capability for determination of glucose in complex samples such as serum. These results suggest that such Janus nanosystems have the potential to construct robust nanoarchitecture with multiple functionalities for various biosensing applications.