Single-Cell Analysis Using Drop-on-Demand Inkjet Printing and Probe Electrospray Ionization Mass Spectrometry

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• 作者：Fengming Chen ; Luyao Lin ; Jie Zhang ; Ziyi He ; Katsumi Uchiyama ; Jin-Ming Lin
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：April 19, 2016
• 年：2016
• 卷：88
• 期：8
• 页码：4354-4360
• 全文大小：455K
• 年卷期：0
• ISSN：1520-6882

文摘

This study describes a novel method for single-cell analysis and lipid profiling by combining drop-on-demand inkjet cell printing and probe electrospray ionization mass spectrometry (PESI-MS). Through inkjet sampling of a cell suspension, droplets with single cells were generated, precisely dripped onto a tungsten-made electrospray ionization needle, and immediately sprayed under a high-voltage electric field. Lipid fingerprints of single cells were obtained by a mass spectrometry (MS) detector. A homemade magnetic stirring device was applied to the cell suspension reservoir, which controlled the homogeneous distribution of cells in liquid and improved the single-cell-droplet percentage by 43.8%. Eight types of single cells were screened in our platform and further differentiated by principal component analysis based on cellular surface phospholipids. Thus, this study successfully provides a facile method for the direct MS profiling of single-cell lipids by PESI-MS.