Characterization by Atomic Force Microscopy of Fused-Silica Capillaries Chemically Modified for Capillary Electrokinetic Chromatography

详细信息    查看全文

• 作者：P. Edward Pullen ; Joseph J. Pesek ; Maria T. Matyska ; and Jane Frommer
• 刊名：Analytical Chemistry
• 出版年：2000
• 出版时间：July 1, 2000
• 年：2000
• 卷：72
• 期：13
• 页码：2751 - 2757
• 全文大小：250K
• 年卷期：v.72,no.13(July 1, 2000)
• ISSN：1520-6882

文摘

Fused-silica capillary tubes with 50-m bores have beenchemically modified to yield octadecyl-derivatized surfaceswith increased surface area. Alkylation is achieved inthree steps: etching, silane adlayering, and olefin hydrosilation. Surface roughness, surface area, surface-tipattractive forces, and topographic images of the native,etched, silane-adlayered, and alkylated capillary bores asmeasured by atomic force microscopy are presented here.Significant roughening occurs during the etching process,but the most dramatic change in roughness and surfaceadhesion forces occurs in the hydrided (silane-adlayered)capillary surface. Topographic smoothing and passivationof surface activity are observed for the alkylated capillarysurface.