Dicyanostilbene-Derived Two-Photon Fluorescence Probe for Free Zinc Ions in Live Cells and Tissues with a Large Two-Photon Action Cross Section

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• 作者：Chibao Huang ; Junle Qu ; Jing Qi ; Meng Yan ; Gaixia Xu
• 刊名：Organic Letters
• 出版年：2011
• 出版时间：March 18, 2011
• 年：2011
• 卷：13
• 期：6
• 页码：1462-1465
• 全文大小：770K
• 年卷期：v.13,no.6(March 18, 2011)
• ISSN：1523-7052

文摘

A novel two-photon fluorescence probe for Zn²⁺ derived from dicyanostilbene as a two-photon fluorophore and 4-(pyridine-2-ylmethyl)piperazine as a novel Zn²⁺ ligand was developed. The probe shows a 72.5-fold fluorescence enhancement in response to Zn²⁺, a large two-photon action cross-section (580 GM), a noncytotoxic effect, and pH insensitivity in the biologically relevant range, and its dissociation constant (K_d^TP) is 0.52 卤 0.01 渭M. The probe can selectively detect free Zn²⁺ ions in live cells for 1500 s or so and in living tissues at a depth of 80鈭?50 渭m without interference from other metal ions and the membrane-bound probes.