Facile Preparation of Biocompatible Sulfhydryl Cotton Fiber-Based Sorbents by 鈥淭hiol鈥揺ne鈥?Click Chemistry for Biological Analysis

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• 作者：Xiao-Mei He ; Gang-Tian Zhu ; Yuan-Yuan Zhu ; Xi Chen ; Zheng Zhang ; Shao-Ting Wang ; Bi-Feng Yuan ; Yu-Qi Feng
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2014
• 出版时间：October 22, 2014
• 年：2014
• 卷：6
• 期：20
• 页码：17857-17864
• 全文大小：369K
• ISSN：1944-8252

文摘

Sulfhydryl cotton fiber (SCF) has been widely used as adsorbent for a variety of metal ions since 1971. Thanks to the abundant thiols on SCF, in this study, we reported a universal method for the facile preparation of SCF-based materials using 鈥渢hiol鈥揺ne鈥?click chemistry for the first time. With the proposed method, two types of SCF-based materials, phenylboronic acid grafted sulfhydryl cotton fiber (SCF-PBA) and zirconium phosphonate-modified sulfhydryl cotton fiber (SCF-pVPA-Zr⁴⁺), were successfully prepared. The grafted functional groups onto the thiol group of SCF were demonstrated by X-ray photoelectron spectroscopy (XPS) and energy dispersive X-ray spectroscopy (EDX). The prepared fibrous materials exhibited excellent fiber strength, good stability in aqueous or nonaqueous solutions, and great biocompatibility. Moreover, we developed filter-free in-pipet-tip SPE using these SCF-based materials as adsorbent for the enrichment of ribonucleosides, glycopeptides and phosphopeptides. Our results showed that SCF-PBA adsorbent can selectively capture ribonucleosides and glycopeptides from complex biological samples. And SCF-pVPA-Zr⁴⁺ adsorbent exhibited high selectivity and capacity in the enrichment of phosphopeptides from the digestion mixture of 尾-casein and bovine serum albumin (BSA), as well as human serum and nonfat milk digest. Generally, the preparation strategy can be a universal method for the synthesis of other functionalized cotton-based adsorbents with special requirement in microscale biological analysis.

Keywords:

sulfhydryl cotton fiber (SCF); 鈥渢hiol鈭抏ne鈥?click chemistry; boronate affinity; immobilized metal ion affinity chromatography (IMAC); biological analysis