Enhanced Bone Marrow Stromal Cell Adhesion and Growth on Segmented Poly(ether ester)s Based on Poly(ethylene oxide) and Poly(butylene terephthalate)

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• 作者：Menno B. Claase ; Mark B. Olde Riekerink ; Joost D. de Bruijn ; Dirk W. Grijpma ; Gerard H. M. Engbers ; and Jan Feijen
• 刊名：Biomacromolecules
• 出版年：2003
• 出版时间：January 2003
• 年：2003
• 卷：4
• 期：1
• 页码：57 - 63
• 全文大小：442K
• 年卷期：v.4,no.1(January 2003)
• ISSN：1526-4602

文摘

In previous studies in rats and goats, hydrophilic compositions of the PEOT/PBT block copolymer familyhave shown in vivo calcification and bone bonding. These copolymers are therefore interesting candidatesas scaffolding materials in bone tissue engineering applications. Model studies using goat bone marrowstromal cells, however, showed that it was not possible to culture bone marrow stromal cells in vitro onthese hydrophilic copolymers. In this paper two ways of surface modifying these materials to improve invitro bone marrow stromal cell attachment and growth are discussed. Two different approaches aredescribed: (1) blending of hydroxyapatite (HA) followed by CO₂ gas plasma etching; (2) surface modificationusing CO₂ gas plasma treatments. It was observed that not only HA but also the CO₂ plasma treatment byitself has a positive effect on bone marrow stromal cell attachment and growth. Gas plasma treatment appearedto be the most successful approach, resulting in a large increase in the amount of bone marrow stromal cellspresent on the surface (determined by a DNA assay). The amount of DNA present on the plasma-treatedcopolymer 1000/70/30 PEOT/PBT, based on poly(ethylene oxide, M_w = 1000, 70 m% soft segment), wascomparable to the amount present on PDLLA and significantly higher than the amount present on PCLafter 7 days of cell culturing. The fact that after gas plasma treatment bone marrow stromal cells do attachto PEOT/PBT copolymers, enables in vitro bone marrow stromal cell culturing, making bone tissueengineering applications of these materials possible.