Development of a Mab-Based Heterologous Immunoassay for the Broad-Selective Determination of Organophosphorus Pesticides

详细信息    查看全文

• 作者：Chunmei Wang ; Xianbin Li ; Yihua Liu ; Yirong Guo ; Rong Xie ; Wenjun Gui ; Guonian Zhu
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2010
• 出版时间：May 12, 2010
• 年：2010
• 卷：58
• 期：9
• 页码：5658-5663
• 全文大小：744K
• 年卷期：v.58,no.9(May 12, 2010)
• ISSN：1520-5118

文摘

A broad-selective monoclonal antibody (Mab) for organophosphorus (OP) pesticides was raised using heterologous indirect enzyme-linked immunosorbent assay (ELISA) to screen hybridomas. On the basis of this Mab, five coating antigens were used to develop homologous and heterologous indirect competitive ELISAs. With the most suitable competitor, a sensitive and broad-selective ELISA was developed. The IC₅₀ values were estimated to be 20.32 ng/mL for parathion, 21.44 ng/mL for methyl-parathion, 42.15 ng/mL for fenitrothion, and 58.85 ng/mL for isocarbophos. Spike recoveries were between 70.52 and 103.27% for the detection of single pesticide residues of the four OP pesticides in purple-clayed paddy soil. Moreover, the chosen ELISA was then applied to the detection of mixtures of parathion and methyl-parathion in soil samples. The average recovery and coefficient of variation were 80.91 and 4.82%, respectively. Results proved that this broad-selective ELISA would be useful for the multiresidue determination of OP pesticides.