Competitive Enzyme-Linked Immunoassay for Sialoglycoprotein of Edible Bird鈥檚 Nest in Food and Cosmetics
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- 作者：Shiwei Zhang ; Xintian Lai ; Xiaoqing Liu ; Yun Li ; Bifang Li ; Xiuli Huang ; Qinlei Zhang ; Wei Chen ; Lin Lin ; Guowu Yang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：April 11, 2012
- 年：2012
- 卷：60
- 期：14
- 页码：3580-3585
- 全文大小：233K
- 年卷期：v.60,no.14(April 11, 2012)
- ISSN：1520-5118
文摘
The proliferation of fake and inferior edible bird鈥檚 nest (EBN) products has recently become an increasingly serious concern. To identify and classify EBN products, a competitive enzyme-linked immunoassay (ELISA) was developed to quantitate sialoglycoprotein in EBN used in food and cosmetic applications. The characteristic sialoglycoprotein in EBN was found, extracted, purified, and analyzed. Sialoglycoprotein, considered the main carrier of sialic acid in EBN, consisted of 106 and 128 kDa proteins. A monoclonal antibody that could recognize both proteins was prepared. The heat-treated process did not change the affinity of sialoglycoprotein with the antibody. An optimized ELISA method was established with a cross-reactivity of less than 0.1% and an IC50 of 3.3 渭g/mL. On the basis of different food and cosmetic samples, the limits of detection (LOD) were 10鈥?8 渭g/g. Recoveries of fortified samples at levels of 20 and 80 渭g/g ranged from 81.5 to 96.5%, respectively. The coefficients of variation were less than 8.0%.