A New Polymer Nanoprobe Based on Chemiluminescence Resonance Energy Transfer for Ultrasensitive Imaging of Intrinsic Superoxide Anion in Mice

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• 作者：Ping Li ; Lu Liu ; Haibin Xiao ; Wei Zhang ; Lulin Wang ; Bo Tang
• 刊名：Journal of the American Chemical Society
• 出版年：2016
• 出版时间：March 9, 2016
• 年：2016
• 卷：138
• 期：9
• 页码：2893-2896
• 全文大小：321K
• ISSN：1520-5126

文摘

Despite significant developments in optical imaging of superoxide anion (O₂^•–) as the preliminary reactive oxygen species, novel visualizing strategies that offer ultrahigh sensitivity are still imperative. This is mainly because intrinsic concentrations of O₂^•– are extremely low in living systems. Herein, we present the rational design and construction of a new polymer nanoprobe PCLA-O₂^•– for detecting O₂^•– based on chemiluminescence (CL) resonance energy transfer without an external excitation source. Structurally, PCLA-O₂^•– contains two moieties linked covalently, namely imidazopyrazinone that is capable of CL triggered by O₂^•– as the energy donor and conjugated polymers with light-amplifying property as the energy acceptor. Experiment results demonstrate that PCLA-O₂^•– exhibits ultrahigh sensitivity at the picomole level, dramatically prolonged luminescence time, specificity, and excellent biocompatibility. Without exogenous stimulation, this probe for the first time in situ visualizes O₂^•– level differences between normal and tumor tissues of mice. These exceptional features ensure that PCLA-O₂^•– as a self-luminescing probe is an alternative in vivo imaging approach for ultralow level O₂^•–.