Covalent Bonding of Heparin on the Crystallized Poly(lactic acid) (PLA) Membrane to Improve Hemocompability via Surface Cross-Linking and Glycidyl Ether Reaction
详细信息 查看全文
- 作者：Zhu Xiong ; Fu Liu ; Haibo Lin ; Jinglong Li ; Yi Wang
- 刊名：ACS Biomaterials Science & Engineering
- 出版年：2016
- 出版时间：December 12, 2016
- 年：2016
- 卷：2
- 期：12
- 页码：2207-2216
- 全文大小：753K
- ISSN：2373-9878
文摘
The hemocompatibility of the dialysis membrane has been considered the key factor influencing the dialysis performance for curing the kidney malfunction. In this work, the commonly used anticoagulant heparin in the clinic was directly bonded to the poly(lactic acid) (PLA) membrane to improve the hemocompatibility via the glycidyl ether reaction. A novel precopolymer P(VP-VTES-GMA) was first prepared via free radical polymerization. The surface cross-linking of the precopolymer containing GMA segments enabled the membrane to anchor heparin through the glycidyl ether reaction. The chemical structure of the precopolymer was confirmed by 1H NMR. The surface chemistry was analyzed by XPS, toluidine blue staining, and solubility tests. The crystallization behavior, heat resistance, morphology, hydrophilicity, pore size, and pure water flux were investigated. The hemocompatibility was comprehensively investigated via APTT, PRT, PT, FIB, and platelet adhesion tests. The simulated dialysis performances relating to urea, creatinine, lysozyme, and BSA clearance were measured. All results demonstrated that the covalent bonding of heparin provided the PLA membrane with excellent hemcompatibility, hydrophilicity, heat resistance, and dialysis performance as well.