Targeting Lipopolyplexes Using Bifunctional Peptides Incorporating Hydrophobic Spacer Amino Acids: Synthesis, Transfection, and Biophysical Studies

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• 作者：Michael A. Pilkington-Miksa ; Michele J. Writer ; Supti Sarkar ; Qing-Hai Meng ; Suzie E. Barker ; Parviz Ayazi Shamlou ; Helen C. Hailes ; Stephen L. Hart ; Alethea B. Tabor
• 刊名：Bioconjugate Chemistry
• 出版年：2007
• 出版时间：November 2007
• 年：2007
• 卷：18
• 期：6
• 页码：1800 - 1810
• 全文大小：814K
• 年卷期：v.18,no.6(November 2007)
• ISSN：1520-4812

文摘

We have developed efficient synthetic routes to two hydrophobic amino acids, suitably protected for solid-phase peptide synthesis, and have successfully synthesized peptides containing these or other hydrophobic amino acids as spacers between a Lys₁₆ moiety and an integrin-targeting motif. These peptides have in turn been used to formulate a range of lipopolyplex vectors with Lipofectin and plasmid DNA. The transfection efficiencies of these vectors and their aggregation behavior in buffers and in serum have been studied. We have shown that vectors containing peptides incorporating long linkers that are entirely hydrophobic are less efficient transfection agents. However, linkers of equivalent length that are in part hydrophobic show improved transfection properties, which is probably due to the improved accessibility of the integrin-binding motif.