Biotin-Fluorophore Conjugates with Poly(ethylene glycol) Spacers Retain Intense Fluorescence after Binding to Avidin and Streptavidin

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• 作者：Hermann J. Gruber ; Markus Marek ; Hansgeorg Schindler ; and Karl Kaiser
• 刊名：Bioconjugate Chemistry
• 出版年：1997
• 出版时间：July 1997
• 年：1997
• 卷：8
• 期：4
• 页码：552 - 559
• 全文大小：262K
• 年卷期：v.8,no.4(July 1997)
• ISSN：1520-4812

文摘

Conventional biotin-fluorophore conjugates with ~14 atom spacerslose most of their fluorescencewhen binding to avidin or streptavidin, as is demonstrated in thepresent study. This explains theunusual fact that only biotinylated marker enzymes, but not fluorescentbiotins, are regularly usedin bioanalytic assays. Novel biotin-spacer-fluorophoreconjugates are presented that retain intensefluorescence when binding to avidin or streptavidin. Preservationof fluorescence depends upon theuse of poly(ethylene glycol) (PEG) spacers, which are shown not tointerfere with biotin function.The observed absence of nonspecific interactions may also beattributed to the PEG chain. Thesenovel fluorescent biotins are expected to be excellent new tools influorescence microscopy and relatedtechniques.