Separation of DNA with Different Configurations on Flat and Nanopatterned Surfaces

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• 作者：Bingquan Li ; Xiaohua Fang ; Haobin Luo ; Young-Soo Seo ; Eric Petersen ; Yuan Ji ; Miriam Rafailovich ; Jonathan Sokolov ; Dilip Gersappe ; and Benjamin Chu
• 刊名：Analytical Chemistry
• 出版年：2006
• 出版时间：July 15, 2006
• 年：2006
• 卷：78
• 期：14
• 页码：4743 - 4751
• 全文大小：550K
• 年卷期：v.78,no.14(July 15, 2006)
• ISSN：1520-6882

文摘

We demonstrate that electrophoresis on a flat Si substrateis an effective method in separation of DNA with differentconfigurations, e.g., linear, supercoiled, and relaxed orDNA of different length, e.g., supercoiled DNA ladder. Thesurface separation arises from the different number ofcontacts due to the conformational differences betweenadsorbed DNA chains. Imposing a Au nanopattern on theSi surface further improves the separation effect. Thesimulation of electric field on this patterned surface bythe finite element method shows that Au nanodots act aslocal pinning points for DNA segments due to dielectrophoretic force. The results of molecular dynamics simulation showed that the conformational differences betweenadsorbed polymer chains were amplified on the patternedsurface and enhanced separations were achieved, whichare consistent with the experimental results.