Development of Atom Transfer Radical Polymer-Modified Gold Nanoparticle-Based Enzyme-Linked Immunosorbent Assay (ELISA)

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• 作者：Feng Chen ; Shike Hou ; Qingsheng Li ; Haojun Fan ; Rong Fan ; Zhongwei Xu ; Gahu Zhala ; Xia Mai ; Xiaoyi Chen ; Xuyi Chen ; Yingfu Liu
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：October 21, 2014
• 年：2014
• 卷：86
• 期：20
• 页码：10021-10024
• 全文大小：293K
• ISSN：1520-6882

文摘

In this work, a novel enzyme-linked immunosorbent assay (ELISA) with a low limit of detection and high sensitivity was developed using atom transfer radical polymer (ATRP)-modified gold nanoparticles (AuNPs). Clear signal amplification was achieved by introducing an abundance of horseradish peroxidase (HRP) to the AuNPs, because of the ATRP modification. This result suggested that the new ELISA was able to detect antigens in complex mixtures, and the limit of detection (LOD) was lower than that of conventional ELISA by a factor of 81. The new ELISA strategy greatly decreased the LOD during analysis and exhibited excellent reproducibility, stability, and feasibility. Therefore, it is a promising technique with many potential applications in biochemistry and medical science research.