Ex Vivo Absorption of Thymol and Thymol-尾-d-glucopyranoside in Piglet Everted Jejunal Segments

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• 作者：Branko T. Petrujki膰 ; Ivana Sedej ; Ross C. Beier ; Robin C. Anderson ; Roger B. Harvey ; Sharon V. R. Epps ; Robert D. Stipanovic ; Nathan A. Krueger ; David J. Nisbet
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：April 17, 2013
• 年：2013
• 卷：61
• 期：15
• 页码：3757-3762
• 全文大小：224K
• 年卷期：v.61,no.15(April 17, 2013)
• ISSN：1520-5118

文摘

Food-producing animals are reservoirs of Campylobacter, a leading bacterial cause of human foodborne illness. The natural product thymol can reduce the survivability of Campylobacter, but its rapid absorption in the proximal gastrointestinal tract may preclude its use as a feed additive to reduce intestinal colonization of these pathogens. This work examined the ex vivo absorption of thymol and thymol-尾-d-glucopyranoside in everted porcine jejunal segments, as the latter was hypothesized to be more resistant to absorption. A modified gas chromatography and extraction method was developed to determine 1.0鈥?00 mg/L thymol. From 1 and 3 mM solutions, 0.293 卤 0.04 and 0.898 卤 0.212 mM thymol, respectively, p = 0.0347, were absorbed, and 0.125 卤 0.041 and 0.317 卤 0.143 mM thymol-尾-d-glucopyranoside, respectively, p = 0.0892, were absorbed. Results indicate that thymol-尾-d-glucopyranoside was absorbed 2.3 to 2.8 times less effectively than thymol, thus providing evidence that thymol-尾-d-glucopyranoside may potentially be used as a feed additive to transport thymol to the piglet lower gut.<br>

Keywords:

gas chromatography; piglet everted small intestine; thymol; thymol-尾-d-glucopyranoside