Bionanoconjugation via Click Chemistry: The Creation of Functional Hybrids of Lipases and Gold Nanoparticles
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- 作者：Jennifer L. Brennan ; Nikos S. Hatzakis ; T. Robert Tshikhudo ; Nijole Dirvianskyte ; Valdemaras Razumas ; Shamkant Patkar ; Jesper Vind ; Allan Svendsen ; Roeland J. M. Nolte ; Alan E. Rowan ; Mathias Brust
- 刊名：Bioconjugate Chemistry
- 出版年：2006
- 出版时间：November 2006
- 年：2006
- 卷：17
- 期：6
- 页码：1373 - 1375
- 全文大小：105K
- 年卷期：v.17,no.6(November 2006)
- ISSN：1520-4812
文摘
A simple and versatile method for the preparation of functional enzyme-gold nanoparticle conjugates using"click" chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalizedThermomyces lanuginosus lipase has been attached to azide-functionalized water-soluble gold nanoparticles underretention of enzymatic activity. The products have been characterized by gel electrophoresis and a fluorometriclipase activity assay. It is estimated that the equivalent of approximately seven fully active lipase molecules areattached to each nanoparticle.