Immunomodulation with Self-Crosslinked Polyelectrolyte Multilayer-Based Coatings

详细信息    查看全文

• 作者：Helena Knopf-Marques ; Sonali Singh ; Su Su Htwe ; Lucie Wolfova ; Radovan Buffa ; Jalal Bacharouche ; Grégory Francius ; Jean-Claude Voegel ; Pierre Schaaf ; Amir M. Ghaemmaghami ; Nihal Engin Vrana ; Philippe Lavalle
• 刊名：Biomacromolecules
• 出版年：2016
• 出版时间：June 13, 2016
• 年：2016
• 卷：17
• 期：6
• 页码：2189-2198
• 全文大小：595K
• 年卷期：0
• ISSN：1526-4602

文摘

This study aims to design an optimal polyelectrolyte multilayer film of poly-l-lysine (PLL) and hyaluronic acid (HA) as an anti-inflammatory cytokine release system in order to decrease the implant failure due to any immune reactions. The chemical modification of the HA with aldehyde moieties allows self-cross-linking of the film and an improvement in the mechanical properties of the film. The cross-linking of the film and the release of immunomodulatory cytokine (IL-4) stimulate the differentiation of primary human monocytes seeded on the films into pro-healing macrophages phenotype. This induces the production of anti-inflammatory cytokines (IL1-RA and CCL18) and the decrease of pro-inflammatory cytokines secreted (IL-12, TNF-α, and IL-1β). Moreover, we demonstrate that cross-linking PLL/HA film using HA-aldehyde is already effective by itself to limit inflammatory processes. Finally, this functionalized self-cross-linked PLL/HA-aldehyde films constitutes an innovative and efficient candidate for immunomodulation of any kind of implants of various architecture and properties.